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SEMA6A Kit ELISA
SEMA6A
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884456
Détail du produit SEMA6A Kit ELISA
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Antigène
Voir toutes SEMA6A Kits ELISA
SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
Reactivité
Toutes les réactivités sur SEMA6A Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human Semaphorin 6A ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes human Semaphorin 6A. Other species not yet determined.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes SEMA6A Kits ELISA
SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
Autre désignation
Semaphorin 6A (SEMA6A Produits )
Synonymes
fj46c12 Kit ELISA, wu:fj46c12 Kit ELISA, zgc:66106 Kit ELISA, SEMA6A Kit ELISA, HT018 Kit ELISA, SEMA Kit ELISA, SEMA6A1 Kit ELISA, SEMAQ Kit ELISA, VIA Kit ELISA, 9330158E07 Kit ELISA, A730020P05Rik Kit ELISA, AI851735 Kit ELISA, Sema6A-1 Kit ELISA, Semaq Kit ELISA, VIa Kit ELISA, b2b997Clo Kit ELISA, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A Kit ELISA, semaphorin 6A Kit ELISA, sema6a Kit ELISA, SEMA6A Kit ELISA, Sema6a Kit ELISA
ID gène
57556
UniProt
Q9H2E6
Vus récemment
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