SEMA7A Kit ELISA

N° du produit ABIN4884468

Détail du produit SEMA7A Kit ELISA

Antigène: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.16-40 ng/mL

Seuil minimal de détection: 0.16 ng/mL

Application: ELISA

Fonction: Human Semaphorin 7A ELISA Kit for serum, plasma, and cell culture supernatant samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ALK-1, B7-H2, BLAME, BMP-8, CD28, Common beta Chain (CD131), Contactin-1, Desmoglein-1, Desmoglein-3, EDAR, EphA1, EphB6, Ephrin-B3, Epiregulin, FGF-12, FGF-17, FOLR2, Galectin-8, Glypican 1, Glypican 5, GHR, IFNgamma R1, IL-22 R alpha 1, IL-22BP, IL-23 R, IL-31 RA, IL-7 R alpha, Integrin alpha 5, MDM2, Nectin-1, NKp30, Nogo Receptor, Notch-3, OSM R beta, Prolactin R, RELT, Ryk, Semaphorin 6D, Siglec-11.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Autre désignation: Semaphorin 7A (SEMA7A Produits)

Synonymes: 2900057C09Rik Kit ELISA, CDw108 Kit ELISA, H-Sema-L Kit ELISA, M-Sema-L Kit ELISA, Semal Kit ELISA, CD108 Kit ELISA, H-SEMA-K1 Kit ELISA, JMH Kit ELISA, SEMAK1 Kit ELISA, SEMAL Kit ELISA, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A Kit ELISA, semaphorin 7A (John Milton Hagen blood group) Kit ELISA, Sema7a Kit ELISA, SEMA7A Kit ELISA

ID gène: 8482

UniProt: O75326

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size