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CHEK2 Kit ELISA

CHEK2 Reactivité: Humain pThr68 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886029
  • Antigène Voir toutes CHEK2 Kits ELISA
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Épitope
    pThr68, total
    Reactivité
    • 6
    • 4
    • 3
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-CHK2 (T68) and Total CHK2 ELISA Kit. This assay semi-quantitatively measures phophorylated CHK2 (Thr68) and Total CHK2 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human CHK2 phosphorylated at site Threonine-68 and Total CHK2.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product CHEK2 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes CHEK2 Kits ELISA
    CHEK2 (Checkpoint Kinase 2 (CHEK2))
    Autre désignation
    CHK2 (CHEK2 Produits)
    Synonymes
    CDS1 Kit ELISA, CHK2 Kit ELISA, HuCds1 Kit ELISA, LFS2 Kit ELISA, PP1425 Kit ELISA, RAD53 Kit ELISA, hCds1 Kit ELISA, fa66f08 Kit ELISA, wu:fa66f08 Kit ELISA, zgc:55865 Kit ELISA, Cds1 Kit ELISA, HUCDS1 Kit ELISA, Rad53 Kit ELISA, Chk2 Kit ELISA, cds1 Kit ELISA, chek2 Kit ELISA, chk2 Kit ELISA, hucds1 Kit ELISA, lfs2 Kit ELISA, pp1425 Kit ELISA, rad53 Kit ELISA, checkpoint kinase 2 Kit ELISA, serine/threonine-protein kinase chk2 Kit ELISA, checkpoint kinase 2 L homeolog Kit ELISA, CHEK2 Kit ELISA, chek2 Kit ELISA, Chek2 Kit ELISA, MCYG_07308 Kit ELISA, chek2.L Kit ELISA
    ID gène
    11200
    UniProt
    O96017
    Pathways
    Signalisation p53, Apoptose, Cycle Cellulaire
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