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MAPK14 Kit ELISA

MAPK14 Reactivité: Humain pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886228
  • Antigène Voir toutes MAPK14 Kits ELISA
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
    Épitope
    pTyr
    Reactivité
    • 16
    • 15
    • 14
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Phosphotyrosine P38 ELISA Kit. This assay semi-quantitatively measures phosphotyrosine P38 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-P38.
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes MAPK14 Kits ELISA
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
    Autre désignation
    p38 (MAPK14 Produits)
    Synonymes
    CSBP Kit ELISA, CSBP1 Kit ELISA, CSBP2 Kit ELISA, CSPB1 Kit ELISA, EXIP Kit ELISA, Mxi2 Kit ELISA, PRKM14 Kit ELISA, PRKM15 Kit ELISA, RK Kit ELISA, SAPK2A Kit ELISA, p38 Kit ELISA, p38ALPHA Kit ELISA, CRK1 Kit ELISA, Csbp1 Kit ELISA, Csbp2 Kit ELISA, Exip Kit ELISA, Hog Kit ELISA, Prkm14 Kit ELISA, Prkm15 Kit ELISA, Sapk2A Kit ELISA, p38Hog Kit ELISA, p38alpha Kit ELISA, p38b Kit ELISA, zp38b Kit ELISA, MAPK14 Kit ELISA, 186F5S Kit ELISA, BG:DS00797.3 Kit ELISA, CG7393 Kit ELISA, D-p38 Kit ELISA, D-p38 MAPK Kit ELISA, D-p38b Kit ELISA, Dm p38b Kit ELISA, Dmel\\CG7393 Kit ELISA, Dmp38b Kit ELISA, Dp38 Kit ELISA, Dp38b Kit ELISA, ESTS:186F5S Kit ELISA, Mpk34C Kit ELISA, anon-sts23 Kit ELISA, dp38b Kit ELISA, p38 MAPK Kit ELISA, p38 beta Kit ELISA, p38B Kit ELISA, p38Kb Kit ELISA, p38beta Kit ELISA, Crk1 Kit ELISA, p38-alpha Kit ELISA, p38MAPK Kit ELISA, p38a Kit ELISA, csbp Kit ELISA, mapk14a Kit ELISA, mxi2 Kit ELISA, sapk2 Kit ELISA, sapk2a Kit ELISA, AP22.98 Kit ELISA, AP22_98 Kit ELISA, ATMPK14 Kit ELISA, mitogen-activated protein kinase 14 Kit ELISA, SAPK2a Kit ELISA, MAP kinase 14A Kit ELISA, MAP kinase p38a Kit ELISA, MAPK 14A Kit ELISA, fk28c03 Kit ELISA, hm:zeh1243 Kit ELISA, wu:fk28c03 Kit ELISA, zp38a Kit ELISA, P38C-CRK Kit ELISA, mitogen-activated protein kinase 14 Kit ELISA, mitogen activated protein kinase 14 Kit ELISA, mitogen-activated protein kinase 14b Kit ELISA, p38b MAP kinase Kit ELISA, mitogen-activated protein kinase 14 S homeolog Kit ELISA, mitogen-activated protein kinase 14a Kit ELISA, CRK proto-oncogene, adaptor protein Kit ELISA, MAPK14 Kit ELISA, Mapk14 Kit ELISA, mapk14b Kit ELISA, p38b Kit ELISA, mapk14.S Kit ELISA, MPK14 Kit ELISA, mapk14a Kit ELISA, CRK Kit ELISA
    ID gène
    1432
    UniProt
    Q16539
    Pathways
    Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
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