Cetuximab Kit ELISA

N° du produit ABIN4886391

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain, Souris et Rat Cetuximab. Il a été cité dans 1 publication.

Aperçu rapide pour Cetuximab Kit ELISA (ABIN4886391)

Antigène: Cetuximab

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 50 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Cetuximab Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Fonction: Quantification of Cetuximab in biological matrices

Analytical Method: Quantitative

Specificité: Cetuximab (Erbitux)

Ingrédients:

• Coated microtiter plate, 96 wells
• Calibrator diluent. - 1.8ml
• Calibrator 12ul 10X wash buffer - 25ml
• Assay buffer - 50ml
• 1000X detection reagent - 17ul TMB - 12ml
• TMB stop solution - 12ml
• Plate sealers - 3

Matériel non inclus:

• Precision pipettes calibrated to deliver 5-1000μL
• Multi-channel pipette calibrated to deliver 50-200μL
• Plate shaker
• Disposable tips
• Vortex-Mixe
• Distilled or de-ionized water
• Microplate reader capable of reading 450nm with background subtraction

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 15 μL

Durée du test: 2.5 h

Plaque: Pre-coated

Protocole: The Cetuximab ELISA kit is designed to measure free Cetuximab with high specificity and sensitivity . This assay employs the sandwich enzyme immunoassay technique. A precoated anti-Cetuximab 96 well plate is provided. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Cetuximab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Cetuximab present in test samples and the concentration is calculated from the standard series.

Préparation des réactifs:

Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation Dilute wash buffer concentrate with deionized water 1/10 before use (for example add 20 mL concentrate to 180 mL deionized water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1/1000 before use (for example add 11 μL concentrate to 11 mL of assay buffer). Mix well. The following is an example calibrator curve.

Prélèvement de l'échantillon: This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20 °C for up to 1 year.

Préparation de l'échantillon:

Dilute calibrators and test samples 1/100 with assay buffer (for example add 5μL of prepared calibrator or sample to 495μL of assay buffer). Mix well. Do not store diluted samples.

Procédure de l'essai:

This assay employs the sandwich enzyme immunoassay technique. Anti- Cetuximab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Cetuximab present in biological matrices is bound by the immobilized anti- Cetuximab antibody. After washing away any unbound substances, enzyme linked anti- Cetuximab antibody is added to the wells. This antibody is developed and purified specifically against truncated Erbitux® (domain residing in Fc portion of the Erbitux® molecule). The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Cetuximab present in test samples. The color development is stopped and the intensity of the color is measured.

Calcul des résultats:

1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used. 2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample. 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with assay buffer and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.

Précision du teste: Intra-assay precision: <10%
Inter-assay precision: <10%

Restrictions: For Research Use only

Stockage

Agent conservateur: Without preservative

Stock: -20 °C

Stockage commentaire: -20C, 1 year

Date de péremption: 12 months

Références pour Cetuximab Kit ELISA (ABIN4886391)

van Helden, Elias, Gerritse, van Es, Boon, Huisman, van Grieken, Dekker, van Dongen, Vugts, Boellaard, van Herpen, de Vries, Oyen, Brouwers, Verheul, Hoekstra, Menke-van der Houven van Oordt: "[89Zr]Zr-cetuximab PET/CT as biomarker for cetuximab monotherapy in patients with RAS wild-type advanced colorectal cancer." dans: European journal of nuclear medicine and molecular imaging, Vol. 47, Issue 4, pp. 849-859, (2021) (PubMed).

Détail du antigène

Antigène: Cetuximab

Sujet: Cetuximab (Erbitux®) is a chimeric IgG1 monoclonal antibody that binds the extra-cellular domain of the epidermal growth factor receptor (EGFR). It is a 152- kDa molecule composed of four polypeptide chains: two identical heavy chains and two identical light chains, consisting of 449 and 214 amino acids, respectively, bound by covalent and non-covalent bonds. The bond with EGFR is characterized by a higher affinity than either endogenous ligand, as epidermal growth factor (EGF), or transforming growth factor alpha. This binding inhibits activation of the receptor tyrosine kinase and the associated downstream signaling that includes the mitogenactivated protein kinase, phosphoinositide 3-kinase/ Akt and the Janus kinases/ signal transducers and activator of transcription (Stat) pathways. Furthermore Cetuximab induces antibody-mediated receptor dimerization, internalization and degradation leading to receptor down-regulation. In addition, it exhibits antibody-dependent cellular cytotoxicity that could contribute to its antitumor effect.

ID gène: 1956