SERPINA3 Kit ELISA

N° du produit ABIN4889786

Détail du produit SERPINA3 Kit ELISA

Antigène: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.137-100 ng/mL

Seuil minimal de détection: 0.137 ng/mL

Application: ELISA

Fonction: Human Serpin A3/Alpha-1-Antichymotrypsin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Serpin A3.

Sensibilité: 0.14 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samples15,000 fold

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SERPINA3 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 3 (SERPINA3))

Autre désignation: Serpin A3 (Alpha-1-Antichymotrypsin) (SERPINA3 Produits)

Synonymes: SERPINA3-1 Kit ELISA, Pi2 Kit ELISA, AACT Kit ELISA, ACT Kit ELISA, GIG25 Kit ELISA, A1AT Kit ELISA, PI Kit ELISA, alpha-1-PI Kit ELISA, AAT Kit ELISA, Pi Kit ELISA, Spi1 Kit ELISA, SERPINA3-5 Kit ELISA, serpina3 Kit ELISA, serpin A3-5 Kit ELISA, serpin family A member 3 Kit ELISA, alpha-1-antiproteinase F precursor Kit ELISA, serpin family A member 1 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 L homeolog Kit ELISA, LOC396684 Kit ELISA, SERPINA3 Kit ELISA, LOC100008973 Kit ELISA, SERPINA1 Kit ELISA, Serpina1 Kit ELISA, SPIA5 Kit ELISA, serpina3.L Kit ELISA

ID gène: 12

UniProt: P01011