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STAT5A Kit ELISA

STAT5A Reactivité: Humain pTyr694 Colorimetric Sandwich ELISA Cell Culture Lysate
N° du produit ABIN4889793
  • Antigène Voir toutes STAT5A Kits ELISA
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Épitope
    pTyr694, total
    Reactivité
    • 8
    • 7
    • 7
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-STAT5A (Tyr694) and Total STAT5A ELISA Kit. This assay semi-quantitatively measures STAT5A phosphorylated at Tyrosine-694 as well as total STAT5A in cell lysate samples.
    Type d'échantillon
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human STAT5A phosphorylated at site Tyrosine-694 as well as total STAT5A.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes STAT5A Kits ELISA
    STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))
    Autre désignation
    STAT5A (STAT5A Produits)
    Synonymes
    MGF Kit ELISA, STAT5 Kit ELISA, AA959963 Kit ELISA, Stat5 Kit ELISA, STAT5A/MGF Kit ELISA, STATA5 Kit ELISA, STAT5A Kit ELISA, stat5 Kit ELISA, stat5b Kit ELISA, signal transducer and activator of transcription 5A Kit ELISA, signal transducer and activator of transcription 5a Kit ELISA, STAT5A Kit ELISA, Stat5a Kit ELISA, stat5a Kit ELISA, LOC100348518 Kit ELISA, LOC100716811 Kit ELISA
    ID gène
    6776
    UniProt
    P42229
    Pathways
    Signalistation JAK/STAT, Signalisation RTK, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation
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