IL-1 beta Kit ELISA

N° du produit ABIN4986941

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris IL-1 beta.

Aperçu rapide pour IL-1 beta Kit ELISA (ABIN4986941)

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.02-1.0 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Détail du produit IL-1 beta Kit ELISA

Seuil minimal de détection: 0.02 ng/mL

Analytical Method: Quantitative

Specificité: Natural and recombinant Mouse IL-1β Ligand

Sensibilité: 7 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Autre désignation: IL-1beta

Sujet: Interleukin 1 (IL-1) is a name that designates two proteins, IL-1α?and IL-1β, which are the products of distinct genes, but which recognize the same cell surface receptors. With the exception of skin keratinocytes, some epithelial cells, and certain cells of the central nervous system, IL-1 is not produced by the cells of healthy individuals. However, in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cell types is seen(1-3).IL-1α ?and IL-1β are structurally related polypeptides that show approximately 25 % homology at the amino acid level (2). Both are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa (4, 5). Intracellular IL-1βconsists exclusively of the 31 kDa precursor form (6). Extracellular IL-1βconsists of a mixture of both unprocessed and mature IL-1β. These results indicate that processing takes place subsequently to secretion and is not tightly coupled to secretion(7). The specific protease apparently responsible for the processing of IL-1β, designated interleukin-1β-converting enzyme (ICE), has been described (7).IL-1 possesses a wide variety of biological activities. IL-1 also plays an important role in immune functions, having effects on macrophages/ onocytes, Tlymphocytes, B lymphocytes, NK cells, and LAK cells. It acts on macrophages/ monocytes, inducing its own synthesis as well as the production of TNF and IL-6 (8,9). It activates T cells, resulting in IL-2 production and expression of IL-2 receptors (10). IL-1 also induces the production of GM-CSF and IL-4 from activated T cells (11). It induces B cell proliferation and maturation and increased immunoglobulin synthesis (12, 13).

Pathways: Signalisation NF-kappaB, Interferon-gamma Pathway, Signalisation TLR, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome