Glutathione Reductase Kit ELISA

N° du produit ABIN4993476

Détail du produit Glutathione Reductase Kit ELISA

Antigène: Glutathione Reductase (GSR)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.63 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.63 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes natural and recombinantRatGR. No significant cross-reactivity or interference between RatGR and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatGR and all the analogues.

Sensibilité: 9.38 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Reference Standard & Sample Diluent
• Biotinylated Detection Antibody (100 x concentrate)
• HRP Conjugate (100 x concentrate)
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Substrate Reagent
• Stop Solution
• Wash Buffer (25 x concentrate)
• Instruction manual

Information d'application

Commentaires:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Volume d'échantillon: 100 µL

Plaque: Pre-coated

Protocole:

1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
3. Aspirate and wash 3 times.
4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
5. Aspirate and wash 5 times.
6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
7. Add 50 µL Stop Solution. Read at 450 nm immediately.
8. Calculation of results.

Préparation des réactifs:

1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 1000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 1000, 500, 250, 125, 62.5, 31.25, 15.63, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 1000 pg/mL working solution to the first tube and mix up to produce a 500 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Stock: 4 °C/-20 °C

Stockage commentaire: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Détail du antigène

Antigène: Glutathione Reductase (GSR)

Autre désignation: Glutathione Reductase (GR) (GSR Produits)

Synonymes: GR Kit ELISA, ATGR2 Kit ELISA, EMB2360 Kit ELISA, glutathione reductase Kit ELISA, AI325518 Kit ELISA, D8Ertd238e Kit ELISA, Gr-1 Kit ELISA, Gr1 Kit ELISA, DDBDRAFT_0168952 Kit ELISA, DDBDRAFT_0231410 Kit ELISA, DDB_0168952 Kit ELISA, DDB_0231410 Kit ELISA, 2151 Kit ELISA, CG2151 Kit ELISA, DTR Kit ELISA, Dm-TrxR Kit ELISA, DmTR Kit ELISA, DmTrx Kit ELISA, DmTrxR Kit ELISA, DmTrxR-1 Kit ELISA, Dmel\\CG2151 Kit ELISA, Gr Kit ELISA, Trx Kit ELISA, TrxR Kit ELISA, TrxR-1 Kit ELISA, Trxr1 Kit ELISA, anon-WO03040301.185 Kit ELISA, anon-WO03040301.187 Kit ELISA, dTrxR Kit ELISA, dmtrxr-1 Kit ELISA, gr Kit ELISA, l(1)G0154 Kit ELISA, l(1)G0379 Kit ELISA, l(1)G0477 Kit ELISA, l(1)G0481 Kit ELISA, trxr-1 Kit ELISA, gor1 Kit ELISA, glutathione reductase, gro-2 Kit ELISA, glutathione reductase Kit ELISA, glutathione-disulfide reductase Kit ELISA, GR; GRase Kit ELISA, mitochondrial glutathione reductase Pgr1 Kit ELISA, Glutathione reductase (GR) (GRase) Kit ELISA, Thioredoxin reductase-1 Kit ELISA, glutathione-disulfide reductase family protein Kit ELISA, glutathione reductase 1 Kit ELISA, GR Kit ELISA, gor Kit ELISA, GSR Kit ELISA, GSR1 Kit ELISA, Gsr_2 Kit ELISA, Gsr Kit ELISA, GSHR1 Kit ELISA, gsr Kit ELISA, LbGR Kit ELISA, pgr1 Kit ELISA, GSHR2 Kit ELISA, GLR2 Kit ELISA, Bcen_2392 Kit ELISA, RPE_1989 Kit ELISA, HCAG_02219 Kit ELISA, SJAG_01162 Kit ELISA, Trxr-1 Kit ELISA, PF14_0192 Kit ELISA, POPTR_0001s14480g Kit ELISA, gsr1 Kit ELISA

Pathways: Thyroid Hormone Synthesis, Cell RedoxHomeostasis