IL-6 Kit ELISA

N° du produit ABIN5026942

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain IL-6.

Aperçu rapide pour IL-6 Kit ELISA (ABIN5026942)

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL-6 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This assay employs an antibody specific for Anti-human IL-6 coated on a 96-well plate.

Analytical Method: Quantitative

Sensibilité: 9.4 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. 

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: IL-6 (IL6) (Interleukin 6 (IL6))

Autre désignation: IL-6 (Interleukin 6)

Pathways: Signalisation TLR, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome