IL-8 Kit ELISA

N° du produit ABIN5026945

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain IL-8.

Aperçu rapide pour IL-8 Kit ELISA (ABIN5026945)

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL-8 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: This assay employs an antibody specific for Anti-human IL-8 coated on a 96-well plate.

Analytical Method: Quantitative

Sensibilité: 18.75 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human IL-8 concentrations in serum, plasma. Detection Range: 31.2 - 2000 pg/mL Sensitivity: < 18.75 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human IL-8 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Autre désignation: IL-8 (Interleukin 8)

Pathways: Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy