IFNA Kit ELISA
Aperçu rapide pour IFNA Kit ELISA (ABIN5026961)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 15.6 pg/mL
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Fonction
- This assay employs an antibody specific for Anti-human IFN-Alphacoated on a 96-well plate.
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Analytical Method
- Quantitative
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Sensibilité
- 9.4 pg/mL
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Attributs du produit
- This immunoassay kit allows for the in vitro quantitative determination of Human IFN-Alpha concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: <9.4 pg/mL
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Ingrédients
- plate, standard, diluent, antibodies
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Matériel non inclus
- pipettes, tubes, reader, incubator
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Volume d'échantillon
- 100 μL
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Durée du test
- 3 - 4 h
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Plaque
- Pre-coated
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Protocole
- This assay employs an antibody specific for Anti-human IFN-Alphacoated on a 96-well plate. Standards and samples are pipetted into the wells and IFN-Alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IFN-Alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN-Alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Restrictions
- For Research Use only
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Précaution d'utilisation
- protect your eyes
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Stock
- 4 °C
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Stockage commentaire
- Please refer to the Manual
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- IFNA (Interferon alpha (IFNA))
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Autre désignation
- IFN Alpha
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Pathways
- Signalistation JAK/STAT, Signalisation TLR, Hepatitis C, Inflammasome
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