IL12 Kit ELISA

N° du produit ABIN5026962

Kit ELISA IL12 Humain, Colorimetric test pour la quantification de Humain IL12.

Aperçu rapide pour IL12 Kit ELISA (ABIN5026962)

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit IL12 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: This kit was based on sandwich enzyme-linked immune-sorbent assay technology.

Analytical Method: Quantitative

Sensibilité: 9.4 pg/mL

Attributs du produit: This immunoassay kit allows for the in vitro quantitative determination of Human IL-12 (p70) concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human IL-12 (p70) antibody was pre-coated into 96-well plates. Biotin conjugated anti-human IL-12 (p70) detection antibody was used. Standards, test samples and biotin conjugated detection antibody were added to the wells subsequently. Wash buffer was used to wash any non-specific binding. HRP conjugated Streptavidin was used as secondary antibody. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-12 (p70) amount of samples captured in the plate. Optical Density (O.D) can be read at absorbance 450 nm in a microplate reader. Concentration of Human IL-12 (p70) can be calculated using the standard curve.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Stockage commentaire: Please refer to the Manual

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: IL-12 (p70) (Interleukin 12, p70)

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome