CD74 Kit ELISA

N° du produit ABIN5067920

Détail du produit CD74 Kit ELISA

Antigène: CD74 (HLA-DR-gamma (CD74))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: The CD74 (HLA Class II Histocompatibility Antigen gamma Chain) ELISA Kit (Human) is a quantitative sandwich assay for the detection of CD74 in human serum, plasma, tissue homogenates and other biological fluids.

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: > 0.375 ng/mL

Attributs du produit: CD74 (HLA Class II Histocompatibility Antigen gamma Chain) ELISA Kit (Human)

Ingrédients:

• *354655A: Microtiter Strips, 8x12 wells.
• *354655B: Standard, 2 vials
• 354655C: Sample/Standard Dilution Buffer, 1x20ml
• 354655D: Antibody-Biotin (Concentrated), 1x120ul
• 354655E: Antibody Dilution Buffer, 1x10ml
• 354655F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 354655G: SABC Dilution Buffer, 1x10ml
• 354655H: TMB Substrate, 1x10ml
• 354655J: Stop Solution, 1x10ml
• 354655K: Wash Buffer (25X), 1x30ml

Information d'application

Plaque: Pre-coated

Protocole: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD74. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to CD74. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD74, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CD74 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Précision du teste: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C/-20°C

Détail du antigène

Antigène: CD74 (HLA-DR-gamma (CD74))

Autre désignation: CD74 (HLA Class II Histocompatibility Antigen gamma Chain) (CD74 Produits)

Synonymes: DHLAG Kit ELISA, HLADG Kit ELISA, II Kit ELISA, Ia-GAMMA Kit ELISA, CLIP Kit ELISA, Ii Kit ELISA, INVG34 Kit ELISA, Iclp-1 Kit ELISA, iclp1 Kit ELISA, wu:fb11f09 Kit ELISA, wu:fb12a01 Kit ELISA, wu:fb13d06 Kit ELISA, wu:fk94e07 Kit ELISA, Iclp-2 Kit ELISA, cd74 Kit ELISA, iclp2 Kit ELISA, zgc:123161 Kit ELISA, CD74 molecule Kit ELISA, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) Kit ELISA, CD74 molecule, major histocompatibility complex, class II invariant chain a Kit ELISA, CD74 molecule, major histocompatibility complex, class II invariant chain b Kit ELISA, CD74 Kit ELISA, Cd74 Kit ELISA, cd74a Kit ELISA, cd74b Kit ELISA

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints