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PDPK1 Kit ELISA

PDPK1 Reactivité: Souris, Humain, Rat pSer241 Colorimetric Cell ELISA Adherent Cell Culture
N° du produit ABIN5526686
  • Antigène Voir toutes PDPK1 Kits ELISA
    PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
    Épitope
    pSer241, total
    Reactivité
    Souris, Humain, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Cell ELISA
    Application
    ELISA
    Fonction
    Cell-Based ELISA Kit. This assay semi-quantitatively measures PDK1 phosphorylated at Serine-241 as well as total PDK1 in adherent cell lines.
    Type d'échantillon
    Adherent Cell Culture
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human, Mouse and Rat PDK1 phosphorylated at site Serine-241 as well as total PDK1.
    Attributs du produit
    • Rapidly measure phosphorylated protein in adherent cell lines
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • No sample lysis is needed
    • Compatible with a standard ELISA plate reader
    Ingrédients
    • Uncoated 96-well Strip Microplate
    • Wash Buffers
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product PDPK1 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes PDPK1 Kits ELISA
    PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))
    Autre désignation
    PDK1 (PDPK1 Produits)
    Synonymes
    pdpk1 Kit ELISA, MGC82080 Kit ELISA, PDK1 Kit ELISA, PDPK2 Kit ELISA, PRO0461 Kit ELISA, Pdk1 Kit ELISA, zgc:153787 Kit ELISA, 3'-phosphoinositide-dependent protein kinase 1 Kit ELISA, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 Kit ELISA, ATPDK1 Kit ELISA, AtPDK1 Kit ELISA, T32M21.110 Kit ELISA, T32M21_110 Kit ELISA, zgc:77318 Kit ELISA, 3-phosphoinositide dependent protein kinase 1 Kit ELISA, 3-phosphoinositide dependent protein kinase 1 L homeolog Kit ELISA, 3-phosphoinositide dependent protein kinase-1 Kit ELISA, 3-phosphoinositide dependent protein kinase 1a Kit ELISA, 3-phosphoinositide-dependent protein kinase 1 Kit ELISA, 3'-phosphoinositide-dependent protein kinase 1 Kit ELISA, 3-phosphoinositide dependent protein kinase 1b Kit ELISA, PDPK1 Kit ELISA, pdpk1.L Kit ELISA, pdpk1 Kit ELISA, Pdpk1 Kit ELISA, pdpk1a Kit ELISA, LOC100380750 Kit ELISA, pdk-1 Kit ELISA, PDK1 Kit ELISA, pdpk1b Kit ELISA
    ID gène
    5170
    UniProt
    O15530, Q9Z2A0, O55173
    Pathways
    Signalisation PI3K-Akt, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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