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PACAP Kit ELISA

ADCYAP1 Reactivité: Humain Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
N° du produit ABIN5651435
  • Antigène Voir toutes PACAP (ADCYAP1) Kits ELISA
    PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))
    Reactivité
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    6.17 pg/mL - 500 pg/mL
    Seuil minimal de détection
    6.17 pg/mL
    Application
    ELISA
    Type d'échantillon
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.
    Sensibilité
    2.55 pg/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    2 h
    Plaque
    Pre-coated
    Protocole
    This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and unlabeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) (Standards or samples) with the pre-coated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes PACAP (ADCYAP1) Kits ELISA
    PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))
    Autre désignation
    Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1 Produits)
    Synonymes
    PACAP Kit ELISA, grf Kit ELISA, Pacap Kit ELISA, adcyap1 Kit ELISA, GRF-PACAP Kit ELISA, zgc:103760 Kit ELISA, xx:af217251 Kit ELISA, pacap Kit ELISA, ADCYAP1 Kit ELISA, PACAP2 Kit ELISA, acap1l Kit ELISA, pacap1b Kit ELISA, wu:fj30e08 Kit ELISA, zgc:101522 Kit ELISA, zgc:92555 Kit ELISA, adenylate cyclase activating polypeptide 1 Kit ELISA, adenylate cyclase activating polypeptide 1a Kit ELISA, adenylate cyclase activating polypeptide 1 (pituitary) Kit ELISA, adenylate cyclase activating polypeptide 1b Kit ELISA, ADCYAP1 Kit ELISA, Adcyap1 Kit ELISA, adcyap1a Kit ELISA, adcyap1 Kit ELISA, adcyap1b Kit ELISA
    Sujet

    Gene Name: Adenylate Cyclase Activating Polypeptide 1, Pituitary

    Gene Aliases: PACAP, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

    ID gène
    116
    UniProt
    P18509
    Pathways
    Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling
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