PACAP Kit ELISA

N° du produit ABIN5651436

Détail du produit PACAP Kit ELISA

Antigène: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.

Sensibilité: 3.2 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PACAP (ADCYAP1) (Adenylate Cyclase Activating Polypeptide 1 (Pituitary) (ADCYAP1))

Autre désignation: Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1 Produits)

Synonymes: PACAP Kit ELISA, grf Kit ELISA, Pacap Kit ELISA, adcyap1 Kit ELISA, GRF-PACAP Kit ELISA, zgc:103760 Kit ELISA, xx:af217251 Kit ELISA, pacap Kit ELISA, ADCYAP1 Kit ELISA, PACAP2 Kit ELISA, acap1l Kit ELISA, pacap1b Kit ELISA, wu:fj30e08 Kit ELISA, zgc:101522 Kit ELISA, zgc:92555 Kit ELISA, adenylate cyclase activating polypeptide 1 Kit ELISA, adenylate cyclase activating polypeptide 1a Kit ELISA, adenylate cyclase activating polypeptide 1 (pituitary) Kit ELISA, adenylate cyclase activating polypeptide 1b Kit ELISA, ADCYAP1 Kit ELISA, Adcyap1 Kit ELISA, adcyap1a Kit ELISA, adcyap1 Kit ELISA, adcyap1b Kit ELISA

Sujet:

Gene Name: Adenylate Cyclase Activating Polypeptide 1, Pituitary

Gene Aliases: PACAP, PRP-48, Pituitary Adenylate Cyclase Activating Peptide

ID gène: 11516

UniProt: O70176

Pathways: Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Activity, cAMP Metabolic Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Regulation of G-Protein Coupled Receptor Protein Signaling