TAP1 Kit ELISA

N° du produit ABIN5651864

Détail du produit TAP1 Kit ELISA

Antigène: TAP1 (Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Antigen Peptide Transporter 1 (TAP1). No significant cross-reactivity or interference between Antigen Peptide Transporter 1 (TAP1) and analogues was observed.

Sensibilité: 0.134 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Antigen Peptide Transporter 1 (TAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Antigen Peptide Transporter 1 (TAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Antigen Peptide Transporter 1 (TAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Antigen Peptide Transporter 1 (TAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antigen Peptide Transporter 1 (TAP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antigen Peptide Transporter 1 (TAP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TAP1 (Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1))

Autre désignation: Antigen Peptide Transporter 1 (TAP1 Produits)

Synonymes: ABC17 Kit ELISA, ABCB2 Kit ELISA, APT1 Kit ELISA, D6S114E Kit ELISA, PSF-1 Kit ELISA, PSF1 Kit ELISA, RING4 Kit ELISA, TAP1*0102N Kit ELISA, TAP1N Kit ELISA, abc17 Kit ELISA, abcb2 Kit ELISA, apt1 Kit ELISA, psf1 Kit ELISA, ring4 Kit ELISA, tap1a Kit ELISA, tap1n Kit ELISA, Abcb2 Kit ELISA, Ham-1 Kit ELISA, Ham1 Kit ELISA, MTP1 Kit ELISA, TAP Kit ELISA, Tap-1 Kit ELISA, Y3 Kit ELISA, Cim Kit ELISA, transporter 1, ATP binding cassette subfamily B member Kit ELISA, transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) L homeolog Kit ELISA, transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) Kit ELISA, TAP1 Kit ELISA, tap1.L Kit ELISA, Tap1 Kit ELISA

Sujet:

Gene Name: Antigen Peptide Transporter 1

Gene Aliases: APT1, ABC17, ABCB2, PSF1, RING4, Y3, TAP1N, Peptide Supply Factor 1, Atp-Binding Cassette,Subfamily B,Member 2, Peptide transporter involved in antigen processing 1

ID gène: 6890

UniProt: Q03518

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process