Aromatase Kit ELISA

N° du produit ABIN5652018

Détail du produit Aromatase Kit ELISA

Antigène: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Seuil minimal de détection: 1.56 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Aromatase (ARO). No significant cross-reactivity or interference between Aromatase (ARO) and analogues was observed.

Sensibilité: 0.62 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aromatase (ARO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aromatase (ARO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aromatase (ARO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aromatase (ARO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aromatase (ARO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aromatase (ARO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Aromatase (CYP19A1) (Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 (CYP19A1))

Autre désignation: Aromatase (CYP19A1 Produits)

Synonymes: ARO Kit ELISA, ARO1 Kit ELISA, CPV1 Kit ELISA, CYAR Kit ELISA, CYP19 Kit ELISA, CYPXIX Kit ELISA, P-450AROM Kit ELISA, Aromatase Kit ELISA, Cyp19 Kit ELISA, Cyp19a Kit ELISA, p450arom Kit ELISA, P450 arom Kit ELISA, aro Kit ELISA, cyp19 Kit ELISA, cyp19a Kit ELISA, p450arom-A Kit ELISA, P450AROM Kit ELISA, Ar Kit ELISA, ArKO Kit ELISA, Int-5 Kit ELISA, Int5 Kit ELISA, CYP19P1 Kit ELISA, CYP19A1 Kit ELISA, CYP19a1 Kit ELISA, P450aromA Kit ELISA, ar1 Kit ELISA, cypXIX Kit ELISA, etID309862.18 Kit ELISA, zgc:136541 Kit ELISA, AroB Kit ELISA, CYP19a2 Kit ELISA, P450aromB Kit ELISA, cyp19b Kit ELISA, zgc:92614 Kit ELISA, cyp19a1 Kit ELISA, cytochrome P450 family 19 subfamily A member 1 Kit ELISA, cytochrome P450, family 19, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450 family 19 subfamily A member 1 L homeolog Kit ELISA, aromatase Kit ELISA, cytochrome P450 aromatase Kit ELISA, cytochrome P450, family 19, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450 19A1 Kit ELISA, cytochrome P450, family 17, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450, family 19 Kit ELISA, cytochrome P450, family 19, subfamily A, polypeptide 1a Kit ELISA, cytochrome P450, family 19, subfamily A, polypeptide 1b Kit ELISA, cytochrome P450 19A1-like Kit ELISA, CYP19A1 Kit ELISA, Cyp19a1 Kit ELISA, cyp19a1.L Kit ELISA, SAV_2383 Kit ELISA, pfcyp19b-tstr Kit ELISA, CYP19 Kit ELISA, cyp19a1a Kit ELISA, cyp19a1b Kit ELISA, LOC101174651 Kit ELISA

Sujet:

Gene Name: Aromatase

Gene Aliases: CYP19A1, CYPXIX, ARO, ARO1, CPV1, CYAR, CYP19, P450AROM, P450-AROM, Estrogen synthase, Cytochrome P450,Family 19,Subfamily A,Polypeptide 1

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis