ARNT Kit ELISA

N° du produit ABIN5652026

Détail du produit ARNT Kit ELISA

Antigène: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). No significant cross-reactivity or interference between Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) and analogues was observed.

Sensibilité: 0.058 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT))

Autre désignation: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT Produits)

Synonymes: HIF-1-beta Kit ELISA, HIF-1beta Kit ELISA, HIF1-beta Kit ELISA, HIF1B Kit ELISA, HIF1BETA Kit ELISA, TANGO Kit ELISA, bHLHe2 Kit ELISA, D3Ertd557e Kit ELISA, Drnt Kit ELISA, ESTM42 Kit ELISA, Hif1b Kit ELISA, W08714 Kit ELISA, mKIAA4051 Kit ELISA, Arnt1 Kit ELISA, ARNT Kit ELISA, zgc:136664 Kit ELISA, arnt1 Kit ELISA, hif1b Kit ELISA, tango Kit ELISA, xArnt Kit ELISA, hif1beta Kit ELISA, hif-1beta Kit ELISA, arnt.2 Kit ELISA, MGC68805 Kit ELISA, aryl hydrocarbon receptor nuclear translocator Kit ELISA, aryl hydrocarbon receptor nuclear translocator S homeolog Kit ELISA, ARNT Kit ELISA, Arnt Kit ELISA, arnt Kit ELISA, arnt.S Kit ELISA

Sujet:

Gene Name: Aryl Hydrocarbon Receptor Nuclear Translocator

Gene Aliases: HIF-1beta, bHLHe2, Hypoxia Inducible Factor 1 Beta, Class E basic helix-loop-helix protein 2, Dioxin receptor, nuclear translocator, Hypoxia-inducible factor 1-beta

ID gène: 405

UniProt: P27540

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, L'effet Warburg