ATM Kit ELISA

N° du produit ABIN5652056

Détail du produit ATM Kit ELISA

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ataxia Telangiectasia Mutated (ATM). No significant cross-reactivity or interference between Ataxia Telangiectasia Mutated (ATM) and analogues was observed.

Sensibilité: 6.4 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ataxia Telangiectasia Mutated (ATM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ataxia Telangiectasia Mutated (ATM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ataxia Telangiectasia Mutated (ATM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ataxia Telangiectasia Mutated (ATM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ataxia Telangiectasia Mutated (ATM) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ataxia Telangiectasia Mutated (ATM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: ATM (Ataxia Telangiectasia Mutated (ATM))

Autre désignation: Ataxia Telangiectasia Mutated (ATM Produits)

Synonymes: ATM Kit ELISA, Atm Kit ELISA, CG6535 Kit ELISA, Dmel\\CG6535 Kit ELISA, Tefu Kit ELISA, atm Kit ELISA, atm/tefu Kit ELISA, dATM Kit ELISA, tef Kit ELISA, Xatm Kit ELISA, at1 Kit ELISA, atdc Kit ELISA, tel1 Kit ELISA, telo1 Kit ELISA, AT1 Kit ELISA, ATA Kit ELISA, ATC Kit ELISA, ATD Kit ELISA, ATDC Kit ELISA, ATE Kit ELISA, TEL1 Kit ELISA, TELO1 Kit ELISA, AI256621 Kit ELISA, C030026E19Rik Kit ELISA, telomere fusion Kit ELISA, ATM serine/threonine kinase L homeolog Kit ELISA, ATM serine/threonine kinase Kit ELISA, ataxia telangiectasia mutated Kit ELISA, ataxia telangiectasia mutated (atm) Kit ELISA, serine/threonine-protein kinase ATM Kit ELISA, tefu Kit ELISA, atm.L Kit ELISA, atm Kit ELISA, ATM Kit ELISA, EDI_100660 Kit ELISA, CpipJ_CPIJ001772 Kit ELISA, BDBG_08252 Kit ELISA, PAAG_02532 Kit ELISA, MCYG_05088 Kit ELISA, VDBG_06833 Kit ELISA, ACLA_015700 Kit ELISA, LOC5565620 Kit ELISA, MGYG_07634 Kit ELISA, PGTG_14279 Kit ELISA, Atm Kit ELISA

Sujet:

Gene Name: Ataxia Telangiectasia Mutated

Gene Aliases: AT1, ATD, ATA, ATC, ATDC, ATE, TEL1, TELO1, Serine-protein kinase ATM

ID gène: 11920

UniProt: Q62388

Pathways: Signalisation p53, Apoptose, Réparation de l'ADN, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus