COL4A1 Kit ELISA (Collagen, Type IV, alpha 1)

Details for Product COL4A1 ELISA Kit No. ABIN5653130
Antigène
  • col4a1
  • wu:fc02g11
  • HANAC
  • ICH
  • POREN1
  • arresten
  • Bru
  • Col4a-1
  • Del(8)44H
  • Raw
  • Svc
  • collagen, type IV, alpha 5 (Alport syndrome)
  • collagen type IV alpha 1 chain
  • collagen, type IV, alpha 1
  • col4a5
  • COL4A1
  • Col4a1
Reactivité
Rat (Rattus)
Alternatives
Kits with alternative reactivity to:
16
14
11
2
2
2
2
1
1
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
3.12 ng/mL - 200 ng/mL
Seuil minimal de détection
3.12 ng/mL
Application
ELISA
Options
Type d'échantillon Plasma, Serum, Tissue Homogenate
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This assay has high sensitivity and excellent specificity for detection of Collagen Type IV Alpha 1 (COL4a1). No significant cross-reactivity or interference between Collagen Type IV Alpha 1 (COL4a1) and analogues was observed.
Sensibilité 1.21 ng/mL
Plasmids, Primers & others Plasmids, Primers & others COL4A1 products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Collagen Type IV Alpha 1 (COL4A1 ELISA Kit Extrait)
Sujet

Gene Name: Collagen Type IV Alpha 1

Gene Aliases: COL4-A1, Arresten, Collagen Alpha-1(IV)chain

Pathways Skeletal Muscle Fiber Development, Growth Factor Binding
Commentaires

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test 3 h
Plaque Pre-coated
Protocole The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type IV Alpha 1 (COL4a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type IV Alpha 1 (COL4a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type IV Alpha 1 (COL4a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type IV Alpha 1 (COL4a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Précision du teste Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type IV Alpha 1 (COL4a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Restrictions For Research Use only
Conseil sur la manipulation The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Stock 4 °C,-20 °C
Stockage commentaire -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
Date de péremption 4-8 months
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