COL5A2 Kit ELISA

N° du produit ABIN5653134

Détail du produit COL5A2 Kit ELISA

Antigène: COL5A2 (Collagen, Type V, alpha 2 (COL5A2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Collagen Type V Alpha 2 (COL5a2). No significant cross-reactivity or interference between Collagen Type V Alpha 2 (COL5a2) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type V Alpha 2 (COL5a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type V Alpha 2 (COL5a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type V Alpha 2 (COL5a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type V Alpha 2 (COL5a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type V Alpha 2 (COL5a2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type V Alpha 2 (COL5a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: COL5A2 (Collagen, Type V, alpha 2 (COL5A2))

Autre désignation: Collagen Type V Alpha 2 (COL5A2 Produits)

Synonymes: COL5A2 Kit ELISA, col5a2l Kit ELISA, im:7038599 Kit ELISA, si:by143e18.1 Kit ELISA, si:dz167c3.3 Kit ELISA, wu:fa13f03 Kit ELISA, wu:fa98d01 Kit ELISA, wu:fj05e03 Kit ELISA, LOC100223323 Kit ELISA, 1110014L14Rik Kit ELISA, D230017N05 Kit ELISA, collagen type V alpha 2 chain Kit ELISA, collagen, type V, alpha 2a Kit ELISA, collagen, type V, alpha 2 Kit ELISA, COL5A2 Kit ELISA, col5a2a Kit ELISA, Col5a2 Kit ELISA

Sujet:

Gene Name: Collagen Type V Alpha 2

Gene Aliases: COL5-A2, Collagen Alpha-2(V)chain, AB Collagen

ID gène: 1290

UniProt: P05997