COL11A1 Kit ELISA

N° du produit ABIN5653149

Détail du produit COL11A1 Kit ELISA

Antigène: COL11A1 (Collagen, Type XI, alpha 1 (COL11A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 39 pg/mL - 2500 pg/mL

Seuil minimal de détection: 39 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Collagen Type XI Alpha 1 (COL11a1). No significant cross-reactivity or interference between Collagen Type XI Alpha 1 (COL11a1) and analogues was observed.

Sensibilité: 15 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type XI Alpha 1 (COL11a1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type XI Alpha 1 (COL11a1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type XI Alpha 1 (COL11a1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type XI Alpha 1 (COL11a1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type XI Alpha 1 (COL11a1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type XI Alpha 1 (COL11a1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: COL11A1 (Collagen, Type XI, alpha 1 (COL11A1))

Autre désignation: Collagen Type XI Alpha 1 (COL11A1 Produits)

Synonymes: wu:fb34e11 Kit ELISA, wu:fc11b03 Kit ELISA, zgc:158335 Kit ELISA, col11a1 Kit ELISA, Col11a1 Kit ELISA, CO11A1 Kit ELISA, COLL6 Kit ELISA, STL2 Kit ELISA, C530001D20Rik Kit ELISA, cho Kit ELISA, collagen type XI alpha 1 chain Kit ELISA, collagen, type XI, alpha 1a Kit ELISA, collagen, type V, alpha 3 Kit ELISA, collagen, type XI, alpha 1 Kit ELISA, COL11A1 Kit ELISA, col11a1a Kit ELISA, col5a3 Kit ELISA, Col11a1 Kit ELISA

Sujet:

Gene Name: Collagen Type XI Alpha 1

Gene Aliases: COL11-A1, CO11A1, COLL6, STL2, Collagen Alpha-1(XI)chain

ID gène: 1301

UniProt: P12107

Pathways: Sensory Perception of Sound