CYP1A1 Kit ELISA

N° du produit ABIN5653494

Détail du produit CYP1A1 Kit ELISA

Antigène: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 1A1 (CYP1A1). No significant cross-reactivity or interference between Cytochrome P450 1A1 (CYP1A1) and analogues was observed.

Sensibilité: 0.129 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1A1 (CYP1A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 1A1 (CYP1A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 1A1 (CYP1A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 1A1 (CYP1A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 1A1 (CYP1A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: CYP1A1 (Cytochrome P450, Family 1, Subfamily A, Polypeptide 1 (CYP1A1))

Autre désignation: Cytochrome P450 1A1 (CYP1A1 Produits)

Synonymes: AHH Kit ELISA, AHRR Kit ELISA, CP11 Kit ELISA, CYP1 Kit ELISA, P1-450 Kit ELISA, P450-C Kit ELISA, P450DX Kit ELISA, Cyp1a2 Kit ELISA, P450-1 Kit ELISA, cyp1a1 Kit ELISA, CYP1A1 Kit ELISA, CYPIA1 Kit ELISA, Cyp45c Kit ELISA, Cypc45c Kit ELISA, P-450MC Kit ELISA, wu:fb63b04 Kit ELISA, zfCYP1A Kit ELISA, zgc:109747 Kit ELISA, ahh Kit ELISA, ahrr Kit ELISA, cp11 Kit ELISA, cyp1 Kit ELISA, cyp1a Kit ELISA, p1-450 Kit ELISA, p450-c Kit ELISA, p450dx Kit ELISA, cytochrome P450 family 1 subfamily A member 1 Kit ELISA, cytochrome P450 1A1 Kit ELISA, cytochrome P450, family 1, subfamily a, polypeptide 1 Kit ELISA, cytochrome P450, family 1, subfamily A, polypeptide 1 Kit ELISA, cytochrome P450 family 1 subfamily D polypeptide 1 Kit ELISA, cytochrome P4501A1 Kit ELISA, polycyclic hydrocarbon-inducible cytochrome P450c Kit ELISA, cytochrome P450, family 1, subfamily A Kit ELISA, cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 1 Kit ELISA, cytochrome P450 family 1 subfamily A member 1 L homeolog Kit ELISA, CYP1A1 Kit ELISA, CpipJ_CPIJ010542 Kit ELISA, Cyp1a1 Kit ELISA, cyp1d1 Kit ELISA, LOC100328613 Kit ELISA, cyp1a Kit ELISA, LOC102129994 Kit ELISA, cyp1a1.L Kit ELISA

Sujet:

Gene Name: Cytochrome P450 1A1

Gene Aliases: CYP1, AHH, AHRR, CP11, CYP1, P1-450, P450-C, P450DX, Cytochrome P450,Subfamily I(Aromatic Compound-Inducible),Polypeptide 1

ID gène: 24296

UniProt: P00185

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha