SLC40A1 Kit ELISA

N° du produit ABIN5654129

Détail du produit SLC40A1 Kit ELISA

Antigène: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ferroportin (FPN). No significant cross-reactivity or interference between Ferroportin (FPN) and analogues was observed.

Sensibilité: 0.131 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferroportin (FPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferroportin (FPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferroportin (FPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferroportin (FPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferroportin (FPN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferroportin (FPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Autre désignation: Ferroportin (SLC40A1 Produits)

Synonymes: FPN1 Kit ELISA, HFE4 Kit ELISA, IREG1 Kit ELISA, MST079 Kit ELISA, MTP1 Kit ELISA, SLC11A3 Kit ELISA, slc39a1 Kit ELISA, weh Kit ELISA, slc40a1 Kit ELISA, MGC82700 Kit ELISA, SLC40A1 Kit ELISA, Fpn1 Kit ELISA, Slc11a3 Kit ELISA, Slc39a1 Kit ELISA, Dusg Kit ELISA, MTP Kit ELISA, Ol5 Kit ELISA, Pcm Kit ELISA, ARABIDOPSIS THALIANA IRON-REGULATED PROTEIN 1 Kit ELISA, ATIREG1 Kit ELISA, FERROPORTIN 1 Kit ELISA, T19C21.5 Kit ELISA, T19C21_5 Kit ELISA, iron regulated 1 Kit ELISA, ARABIDOPSIS IRON-REGULATED TRANSPORTER 1 Kit ELISA, ATIRT1 Kit ELISA, T16H5.50 Kit ELISA, T16H5_50 Kit ELISA, iron-regulated transporter 1 Kit ELISA, solute carrier family 40 member 1 Kit ELISA, solute carrier family 40 (iron-regulated transporter), member 1 Kit ELISA, solute carrier family 40 (iron-regulated transporter), member 1 S homeolog Kit ELISA, iron regulated 1 Kit ELISA, iron-regulated transporter 1 Kit ELISA, SLC40A1 Kit ELISA, slc40a1 Kit ELISA, slc40a1.S Kit ELISA, Slc40a1 Kit ELISA, IREG1 Kit ELISA, IRT1 Kit ELISA

Sujet:

Gene Name: Ferroportin

Gene Aliases: SLC40A1, FPN1, HFE4, IREG1, MTP1, SLC11A3, Solute Carrier Family 40,Member 1, Iron-Regulated Transporter

ID gène: 30061

UniProt: Q9NP59

Pathways: Transition Metal Ion Homeostasis