GRB2 Kit ELISA

N° du produit ABIN5654877

Détail du produit GRB2 Kit ELISA

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 2 (Grb2). No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 2 (Grb2) and analogues was observed.

Sensibilité: 0.055 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Factor Receptor Bound Protein 2 (Grb2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Factor Receptor Bound Protein 2 (Grb2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Factor Receptor Bound Protein 2 (Grb2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Factor Receptor Bound Protein 2 (Grb2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 2 (Grb2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 2 (Grb2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Autre désignation: Growth Factor Receptor Bound Protein 2 (GRB2 Produits)

Synonymes: GRB2 Kit ELISA, grb2 Kit ELISA, AA408164 Kit ELISA, Ash Kit ELISA, ASH Kit ELISA, EGFRBP-GRB2 Kit ELISA, Grb3-3 Kit ELISA, MST084 Kit ELISA, MSTP084 Kit ELISA, NCKAP2 Kit ELISA, wu:fb18e12 Kit ELISA, wu:fb34e06 Kit ELISA, zgc:73077 Kit ELISA, growth factor receptor bound protein 2 Kit ELISA, growth factor receptor-bound protein 2 Kit ELISA, growth factor receptor-bound protein 2b Kit ELISA, GRB2 Kit ELISA, grb2 Kit ELISA, Grb2 Kit ELISA, grb2b Kit ELISA

Sujet:

Gene Name: Growth Factor Receptor Bound Protein 2

Gene Aliases: ASH, NCKAP2, EGFRBP-GRB2, Grb3-3, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2

ID gène: 2885

UniProt: P62993

Pathways: Signalisation RTK, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation