Growth Hormone Receptor Kit ELISA
-
- Antigène Voir toutes Growth Hormone Receptor (GHR) Kits ELISA
- Growth Hormone Receptor (GHR)
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78.12 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 78.12 pg/mL
- Application
- ELISA
- Type d'échantillon
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Growth Hormone Receptor (GHR). No significant cross-reactivity or interference between Growth Hormone Receptor (GHR) and analogues was observed.
- Sensibilité
- 34 pg/mL
- Featured
- Discover our best selling GHR Kit ELISA
- Top Product
- Discover our top product GHR Kit ELISA
-
-
- Commentaires
-
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone Receptor (GHR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone Receptor (GHR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone Receptor (GHR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone Receptor (GHR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
-
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone Receptor (GHR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Hormone Receptor (GHR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
-
- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
-
- Antigène Voir toutes Growth Hormone Receptor (GHR) Kits ELISA
- Growth Hormone Receptor (GHR)
- Autre désignation
- Growth Hormone Receptor (GHR Produits)
- Synonymes
- GHBP Kit ELISA, AA986417 Kit ELISA, GHR/BP Kit ELISA, GHR Kit ELISA, ghr Kit ELISA, ghr.a Kit ELISA, zgc:162141 Kit ELISA, growth hormone receptor Kit ELISA, growth hormone receptor L homeolog Kit ELISA, growth hormone receptor a Kit ELISA, GHR Kit ELISA, Ghr Kit ELISA, ghr.L Kit ELISA, ghr Kit ELISA, ghra Kit ELISA
- Sujet
-
Gene Name: Growth Hormone Receptor
Gene Aliases: GHBP, Somatotropin receptor, Growth hormone-binding protein, Serum-binding protein
- ID gène
- 14600
- UniProt
- P16882
- Pathways
- Signalisation NF-kappaB, Signalistation JAK/STAT, Response to Growth Hormone Stimulus
-