HSP27 Kit ELISA

N° du produit ABIN5654951

Détail du produit HSP27 Kit ELISA

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Heat Shock 27 kDa Protein 1 (HSPB1). No significant cross-reactivity or interference between Heat Shock 27 kDa Protein 1 (HSPB1) and analogues was observed.

Sensibilité: 0.26 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 27 kDa Protein 1 (HSPB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 27 kDa Protein 1 (HSPB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 27 kDa Protein 1 (HSPB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 27 kDa Protein 1 (HSPB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heat Shock 27kDa Protein 1 (HSPB1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heat Shock 27kDa Protein 1 (HSPB1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: Heat Shock 27kDa Protein 1 (HSPB1 Produits)

Synonymes: CMT2F Kit ELISA, HMN2B Kit ELISA, HS.76067 Kit ELISA, HSP27 Kit ELISA, HSP28 Kit ELISA, Hsp25 Kit ELISA, SRP27 Kit ELISA, 27kDa Kit ELISA, Hsp27 Kit ELISA, cb153 Kit ELISA, cb660 Kit ELISA, hsp1 Kit ELISA, hsp25 Kit ELISA, hsp27 Kit ELISA, id:ibd2821 Kit ELISA, sb:cb660 Kit ELISA, zgc:103437 Kit ELISA, HSPB1 Kit ELISA, cmt2f Kit ELISA, hs.76067 Kit ELISA, hsp28 Kit ELISA, heat shock protein family B (small) member 1 Kit ELISA, heat shock protein 1 Kit ELISA, heat shock protein, alpha-crystallin-related, 1 Kit ELISA, heat shock protein family B (small) member 1 L homeolog Kit ELISA, HSPB1 Kit ELISA, Hspb1 Kit ELISA, hspb1 Kit ELISA, hspb1.L Kit ELISA

Sujet:

Gene Name: Heat Shock 27 kDa Protein 1

Gene Aliases: HSP27, HSP-B1, CMT2F, HSP28, Hsp25, Heat Shock Protein 27, 28 kDa heat shock protein, Heat shock protein beta-1, Estrogen-regulated 24 kDa protein,

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling