HBEGF Kit ELISA
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- Antigène Voir toutes HBEGF Kits ELISA
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay has high sensitivity and excellent specificity for detection of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). No significant cross-reactivity or interference between Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) and analogues was observed.
- Sensibilité
- 6.1 pg/mL
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- Commentaires
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Durée du test
- 3 h
- Plaque
- Pre-coated
- Protocole
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Précision du teste
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Conseil sur la manipulation
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Date de péremption
- 4-8 months
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- Antigène Voir toutes HBEGF Kits ELISA
- HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
- Autre désignation
- Heparin Binding Epidermal Growth Factor Like Growth Factor (HBEGF Produits)
- Synonymes
- hbegf Kit ELISA, zgc:165443 Kit ELISA, HBEGF Kit ELISA, zgc:175091 Kit ELISA, DTR Kit ELISA, DTS Kit ELISA, DTSF Kit ELISA, HEGFL Kit ELISA, Dtr Kit ELISA, GFHB Kit ELISA, Hb-egf Kit ELISA, Hegfl Kit ELISA, AW047313 Kit ELISA, Dts Kit ELISA, HB-EGF Kit ELISA, heparin-binding EGF-like growth factor b Kit ELISA, heparin binding EGF like growth factor Kit ELISA, Heparin-binding EGF-like growth factor Kit ELISA, heparin-binding EGF-like growth factor a Kit ELISA, heparin-binding EGF-like growth factor Kit ELISA, hbegfb Kit ELISA, HBEGF Kit ELISA, hbegf Kit ELISA, hbegfa Kit ELISA, Hbegf Kit ELISA
- Sujet
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Gene Name: Heparin Binding Epidermal Growth Factor Like Growth Factor
Gene Aliases: HB-EGF, DTR, DTS, DTSF, HEGFL, Diphtheria Toxin Receptor, Proheparin-binding EGF-like growth factor, Diphtheria toxin receptor
- ID gène
- 15200
- UniProt
- Q06186
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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