Insulin Receptor Kit ELISA

N° du produit ABIN5655463

Détail du produit Insulin Receptor Kit ELISA

Antigène: Insulin Receptor (INSR)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Insulin Receptor (ISR). No significant cross-reactivity or interference between Insulin Receptor (ISR) and analogues was observed.

Sensibilité: 0.34 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Receptor (ISR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Receptor (ISR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Receptor (ISR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Insulin Receptor (ISR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin Receptor (ISR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin Receptor (ISR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Insulin Receptor (INSR)

Autre désignation: Insulin Receptor (INSR Produits)

Synonymes: CD220 Kit ELISA, HHF5 Kit ELISA, 4932439J01Rik Kit ELISA, D630014A15Rik Kit ELISA, IR Kit ELISA, IR-A Kit ELISA, IR-B Kit ELISA, 18402 Kit ELISA, CG18402 Kit ELISA, DIHR Kit ELISA, DILR Kit ELISA, DIR Kit ELISA, DIRH Kit ELISA, DIRbeta Kit ELISA, DInR Kit ELISA, DInr Kit ELISA, Dir-a Kit ELISA, Dir-b Kit ELISA, Dmel\\CG18402 Kit ELISA, INR Kit ELISA, INS Kit ELISA, Inr Kit ELISA, Inr-alpha Kit ELISA, Inr-beta Kit ELISA, InsR Kit ELISA, dINR Kit ELISA, dIR Kit ELISA, dIRH Kit ELISA, dInR Kit ELISA, dInr Kit ELISA, dInsR Kit ELISA, dinr Kit ELISA, dir Kit ELISA, er10 Kit ELISA, inr Kit ELISA, insulin/insulin-like growth factor receptor Kit ELISA, l(3)05545 Kit ELISA, l(3)93Dj Kit ELISA, l(3)er10 Kit ELISA, lnR Kit ELISA, ir-A Kit ELISA, CTK-1 Kit ELISA, ir Kit ELISA, INSR Kit ELISA, NV14476 Kit ELISA, cd220 Kit ELISA, hhf5 Kit ELISA, insulin receptor Kit ELISA, Insulin-like receptor Kit ELISA, insulin receptor L homeolog Kit ELISA, INSR Kit ELISA, Insr Kit ELISA, InR Kit ELISA, LOC100122567 Kit ELISA, LOC100451802 Kit ELISA, insr.L Kit ELISA

Sujet:

Gene Name: Insulin Receptor

Gene Aliases: CD220, INSR, HHF5, IR

UniProt: P15127

Pathways: Signalisation NF-kappaB, Signalisation RTK, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity