IL12 Kit CLIA

N° du produit ABIN5655751

Détail du produit IL12 Kit CLIA

Antigène: IL12 (Interleukin 12 (IL12))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.81 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Interleukin 12 (IL12). No significant cross-reactivity or interference between Interleukin 12 (IL12) and analogues was observed.

Sensibilité: 0.28 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 (IL12). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 12 (IL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 12 (IL12) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 12 (IL12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IL12 (Interleukin 12 (IL12))

Autre désignation: Interleukin 12 (IL12 Produits)

Synonymes: CLMF Kit CLIA, CLMF2 Kit CLIA, IL-12B Kit CLIA, NKSF Kit CLIA, NKSF2 Kit CLIA, p40 Kit CLIA, Il-12b Kit CLIA, Il12p40 Kit CLIA, Il-12p40 Kit CLIA, Il12 Kit CLIA, IL-12p40 Kit CLIA, IL-12 Kit CLIA, LOC100217488 Kit CLIA, IL-12p35 Kit CLIA, Il-12a Kit CLIA, Ll12a Kit CLIA, p35 Kit CLIA, IL12p40 Kit CLIA, IL12B Kit CLIA, IL-12 p35 Kit CLIA, interleukin 12B Kit CLIA, interleukin 12A Kit CLIA, interleukin 12b Kit CLIA, interleukin 12a Kit CLIA, Interleukin 12 level Kit CLIA, IL12B Kit CLIA, IL12A Kit CLIA, Il12b Kit CLIA, Il12a Kit CLIA, IL12 Kit CLIA, il12a Kit CLIA

Sujet:

Gene Name: Interleukin 12

Pathways: Signalistation JAK/STAT, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome