IL-8 Kit CLIA

N° du produit ABIN5656057

Détail du produit IL-8 Kit CLIA

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.62 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8). No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.

Sensibilité: 0.57 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 (IL8). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 8 (IL8) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 8 (IL8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: IL-8 (IL8) (Interleukin 8 (IL8))

Autre désignation: Interleukin 8 (IL8 Produits)

Synonymes: CXCL8 Kit CLIA, GCP-1 Kit CLIA, GCP1 Kit CLIA, LECT Kit CLIA, LUCT Kit CLIA, LYNAP Kit CLIA, MDNCF Kit CLIA, MONAP Kit CLIA, NAF Kit CLIA, NAP-1 Kit CLIA, NAP1 Kit CLIA, IL-8 Kit CLIA, AMCF-I Kit CLIA, il8 Kit CLIA, IL8 Kit CLIA, si:dkey-151b16.2 Kit CLIA, cxcli2 Kit CLIA, cxcl8 Kit CLIA, gcp-1 Kit CLIA, il-8 Kit CLIA, lynap Kit CLIA, mdncf Kit CLIA, monap Kit CLIA, nap-1 Kit CLIA, C-X-C motif chemokine ligand 8 Kit CLIA, interleukin 8 Kit CLIA, chemokine (C-X-C motif) ligand 8a Kit CLIA, chemokine (C-X-C motif) ligand 1-like Kit CLIA, chemokine (C-X-C motif) ligand 8 L homeolog Kit CLIA, CXCL8 Kit CLIA, il8 Kit CLIA, cxcl8a Kit CLIA, IL8 Kit CLIA, Cxcl8 Kit CLIA, LOC422654 Kit CLIA, cxcl8.L Kit CLIA

Sujet:

Gene Name: Interleukin 8

Gene Aliases: CXCL8, AMCF-I, GCP1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP1, SCYB8, TSG1, B-ENAP, Neutrophil-Activating Protein 1, Granulocyte Chemotactic Protein 1

ID gène: 3576

Pathways: Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy