PSMB9 Kit ELISA

N° du produit ABIN5656322

Détail du produit PSMB9 Kit ELISA

Antigène: PSMB9 (Proteasome (Prosome, Macropain) Subunit, beta Type, 9 (Large Multifunctional Peptidase 2) (PSMB9))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Large Multifunctional Peptidase 2 (LMP2). No significant cross-reactivity or interference between Large Multifunctional Peptidase 2 (LMP2) and analogues was observed.

Sensibilité: 0.051 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Multifunctional Peptidase 2 (LMP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Large Multifunctional Peptidase 2 (LMP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Multifunctional Peptidase 2 (LMP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Large Multifunctional Peptidase 2 (LMP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Large Multifunctional Peptidase 2 (LMP2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Large Multifunctional Peptidase 2 (LMP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PSMB9 (Proteasome (Prosome, Macropain) Subunit, beta Type, 9 (Large Multifunctional Peptidase 2) (PSMB9))

Autre désignation: Large Multifunctional Peptidase 2 (PSMB9 Produits)

Synonymes: LMP2 Kit ELISA, ring12 Kit ELISA, MHC-linked Kit ELISA, psmb9b Kit ELISA, lmp2 Kit ELISA, lmp2b Kit ELISA, psmb9 Kit ELISA, Lmp-2 Kit ELISA, Lmp2 Kit ELISA, PSMB6i Kit ELISA, RING12 Kit ELISA, beta1i Kit ELISA, psmb9-A Kit ELISA, macropain Kit ELISA, prosome Kit ELISA, wu:fa66f04 Kit ELISA, wu:fj22d05 Kit ELISA, zgc:91842 Kit ELISA, psmb9a Kit ELISA, proteasome subunit beta 9 Kit ELISA, LMP2 Kit ELISA, proteasome subunit beta type-9b Kit ELISA, proteasome (prosome, macropain) subunit, beta type 9 (large multifunctional peptidase 2) Kit ELISA, proteasome subunit beta 9 S homeolog Kit ELISA, proteasome subunit beta 9a Kit ELISA, proteasome (prosome, macropain) subunit, beta type, 9a Kit ELISA, psmb9 Kit ELISA, PSMB9 Kit ELISA, psmb9-b Kit ELISA, Psmb9 Kit ELISA, psmb9.S Kit ELISA, psmb9a Kit ELISA

Sujet:

Gene Name: Large Multifunctional Peptidase 2

Gene Aliases: PSMB9, Proteasome(Prosome,Macropain)Subunit Beta Type 9, Macropain chain 7, Multicatalytic endopeptidase complex chain 7, Really interesting new gene 12 protein

ID gène: 5698

UniProt: P28065

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA