MAPK12 Kit ELISA

N° du produit ABIN5656922

Détail du produit MAPK12 Kit ELISA

Antigène: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase 12 (MAPK12). No significant cross-reactivity or interference between Mitogen Activated Protein Kinase 12 (MAPK12) and analogues was observed.

Sensibilité: 0.072 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 12 (MAPK12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase 12 (MAPK12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitogen Activated Protein Kinase 12 (MAPK12) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitogen Activated Protein Kinase 12 (MAPK12) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Autre désignation: Mitogen Activated Protein Kinase 12 (MAPK12 Produits)

Synonymes: ATMPK12 Kit ELISA, MAPK12 Kit ELISA, T3F17.28 Kit ELISA, mitogen-activated protein kinase 12 Kit ELISA, MGC160082 Kit ELISA, si:dkey-14d8.5 Kit ELISA, erk6 Kit ELISA, etID309866.18 Kit ELISA, mapk12 Kit ELISA, sapk3 Kit ELISA, wu:fa05c12 Kit ELISA, zgc:101695 Kit ELISA, ERK3 Kit ELISA, ERK6 Kit ELISA, P38GAMMA Kit ELISA, PRKM12 Kit ELISA, SAPK-3 Kit ELISA, SAPK3 Kit ELISA, AW123708 Kit ELISA, Erk6 Kit ELISA, P38gamma Kit ELISA, Prkm12 Kit ELISA, Sapk3 Kit ELISA, mitogen-activated protein kinase 12 Kit ELISA, mitogen-activated protein kinase 12b Kit ELISA, mitogen-activated protein kinase 12a Kit ELISA, MPK12 Kit ELISA, MAPK12 Kit ELISA, mapk12b Kit ELISA, mapk12a Kit ELISA, Mapk12 Kit ELISA

Sujet:

Gene Name: Mitogen Activated Protein Kinase 12

Gene Aliases: ERK6, PRKM12, p38 Gamma, SAPK3, Stress-activated protein kinase 3, Extracellular signal-regulated kinase 6, Mitogen-activated protein kinase p38 gamma

ID gène: 6300

UniProt: P53778

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins