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MAPK14 Kit ELISA

MAPK14 Reactivité: Rat Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Tissue Homogenate
N° du produit ABIN5656925
  • Antigène Voir toutes MAPK14 Kits ELISA
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
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    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.25 ng/mL - 80 ng/mL
    Seuil minimal de détection
    1.25 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Mitogen Activated Protein Kinase 14 (MAPK14). No significant cross-reactivity or interference between Mitogen Activated Protein Kinase 14 (MAPK14) and analogues was observed.
    Sensibilité
    0.43 ng/mL
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  • Commentaires

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 14 (MAPK14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 14 (MAPK14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 14 (MAPK14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mitogen Activated Protein Kinase 14 (MAPK14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mitogen Activated Protein Kinase 14 (MAPK14) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mitogen Activated Protein Kinase 14 (MAPK14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Date de péremption
    4-8 months
  • Antigène Voir toutes MAPK14 Kits ELISA
    MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))
    Autre désignation
    Mitogen Activated Protein Kinase 14 (MAPK14 Produits)
    Synonymes
    CSBP Kit ELISA, CSBP1 Kit ELISA, CSBP2 Kit ELISA, CSPB1 Kit ELISA, EXIP Kit ELISA, Mxi2 Kit ELISA, PRKM14 Kit ELISA, PRKM15 Kit ELISA, RK Kit ELISA, SAPK2A Kit ELISA, p38 Kit ELISA, p38ALPHA Kit ELISA, CRK1 Kit ELISA, Csbp1 Kit ELISA, Csbp2 Kit ELISA, Exip Kit ELISA, Hog Kit ELISA, Prkm14 Kit ELISA, Prkm15 Kit ELISA, Sapk2A Kit ELISA, p38Hog Kit ELISA, p38alpha Kit ELISA, p38b Kit ELISA, zp38b Kit ELISA, MAPK14 Kit ELISA, 186F5S Kit ELISA, BG:DS00797.3 Kit ELISA, CG7393 Kit ELISA, D-p38 Kit ELISA, D-p38 MAPK Kit ELISA, D-p38b Kit ELISA, Dm p38b Kit ELISA, Dmel\\CG7393 Kit ELISA, Dmp38b Kit ELISA, Dp38 Kit ELISA, Dp38b Kit ELISA, ESTS:186F5S Kit ELISA, Mpk34C Kit ELISA, anon-sts23 Kit ELISA, dp38b Kit ELISA, p38 MAPK Kit ELISA, p38 beta Kit ELISA, p38B Kit ELISA, p38Kb Kit ELISA, p38beta Kit ELISA, Crk1 Kit ELISA, p38-alpha Kit ELISA, p38MAPK Kit ELISA, p38a Kit ELISA, csbp Kit ELISA, mapk14a Kit ELISA, mxi2 Kit ELISA, sapk2 Kit ELISA, sapk2a Kit ELISA, AP22.98 Kit ELISA, AP22_98 Kit ELISA, ATMPK14 Kit ELISA, mitogen-activated protein kinase 14 Kit ELISA, SAPK2a Kit ELISA, MAP kinase 14A Kit ELISA, MAP kinase p38a Kit ELISA, MAPK 14A Kit ELISA, fk28c03 Kit ELISA, hm:zeh1243 Kit ELISA, wu:fk28c03 Kit ELISA, zp38a Kit ELISA, P38C-CRK Kit ELISA, mitogen-activated protein kinase 14 Kit ELISA, mitogen activated protein kinase 14 Kit ELISA, mitogen-activated protein kinase 14b Kit ELISA, p38b MAP kinase Kit ELISA, mitogen-activated protein kinase 14 S homeolog Kit ELISA, mitogen-activated protein kinase 14a Kit ELISA, CRK proto-oncogene, adaptor protein Kit ELISA, MAPK14 Kit ELISA, Mapk14 Kit ELISA, mapk14b Kit ELISA, p38b Kit ELISA, mapk14.S Kit ELISA, MPK14 Kit ELISA, mapk14a Kit ELISA, CRK Kit ELISA
    Sujet

    Gene Name: Mitogen Activated Protein Kinase 14

    Gene Aliases: CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, P38, Mitogen-activated protein kinase p38 alpha, Cytokine suppressive anti-inflammatory drug-binding protein

    Pathways
    Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins
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