Paxillin Kit ELISA

N° du produit ABIN5657652

Détail du produit Paxillin Kit ELISA

Antigène: Paxillin (PXN)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Paxillin (Pax). No significant cross-reactivity or interference between Paxillin (Pax) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Paxillin (Pax). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Paxillin (Pax). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Paxillin (Pax), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Paxillin (Pax) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Paxillin (Pax) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Paxillin (Pax) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: Paxillin (PXN)

Autre désignation: Paxillin (PXN Produits)

Synonymes: AW108311 Kit ELISA, AW123232 Kit ELISA, Pax Kit ELISA, CG18061 Kit ELISA, CG18576 Kit ELISA, CG31794 Kit ELISA, CT40481 Kit ELISA, CT42454 Kit ELISA, DPaxillin Kit ELISA, DPxn Kit ELISA, DPxn37 Kit ELISA, Dmel\\CG31794 Kit ELISA, Dpax Kit ELISA, DpaxA Kit ELISA, PDLP Kit ELISA, dPax Kit ELISA, pax Kit ELISA, wu:fw71f12 Kit ELISA, PXN Kit ELISA, LOC100220858 Kit ELISA, paxillin Kit ELISA, Paxillin Kit ELISA, paxillin S homeolog Kit ELISA, paxillin a Kit ELISA, PXN Kit ELISA, Pxn Kit ELISA, Pax Kit ELISA, pxn.S Kit ELISA, pxna Kit ELISA, LOC696336 Kit ELISA, pxn Kit ELISA

Sujet:

Gene Name: Paxillin

Gene Aliases: PXN

ID gène: 19303

UniProt: Q8VI36

Pathways: Signalisation MAPK, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling