PTGS2 Kit ELISA

N° du produit ABIN5658150

Détail du produit PTGS2 Kit ELISA

Antigène: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Prostaglandin Endoperoxide Synthase 2 (PTGS2). No significant cross-reactivity or interference between Prostaglandin Endoperoxide Synthase 2 (PTGS2) and analogues was observed.

Sensibilité: 0.065 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prostaglandin Endoperoxide Synthase 2 (PTGS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prostaglandin Endoperoxide Synthase 2 (PTGS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prostaglandin Endoperoxide Synthase 2 (PTGS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prostaglandin Endoperoxide Synthase 2 (PTGS2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PTGS2 (Prostaglandin-Endoperoxide Synthase 2 (Prostaglandin G/H Synthase and Cyclooxygenase) (PTGS2))

Autre désignation: Prostaglandin Endoperoxide Synthase 2 (PTGS2 Produits)

Synonymes: COX-2 Kit ELISA, COX2 Kit ELISA, GRIPGHS Kit ELISA, PGG/HS Kit ELISA, PGHS-2 Kit ELISA, PHS-2 Kit ELISA, hCox-2 Kit ELISA, Cox-2 Kit ELISA, Pghs2 Kit ELISA, TIS10 Kit ELISA, Cox2 Kit ELISA, cox-2 Kit ELISA, CEF147 Kit ELISA, PGHS2 Kit ELISA, PHSII Kit ELISA, cox2 Kit ELISA, gripghs Kit ELISA, pgg/hs Kit ELISA, pghs-2 Kit ELISA, phs-2 Kit ELISA, PTGS2 Kit ELISA, hcox-2 Kit ELISA, fj02a10 Kit ELISA, ptgs2 Kit ELISA, unp1239 Kit ELISA, wu:fj02a10 Kit ELISA, zCOX-2 Kit ELISA, si:dkey-97o5.6 Kit ELISA, prostaglandin-endoperoxide synthase 2 Kit ELISA, prostaglandin G/H synthase 2 Kit ELISA, prostaglandin-endoperoxide synthase 2S homeolog Kit ELISA, prostaglandin-endoperoxide synthase 2a Kit ELISA, prostaglandin-endoperoxide synthase 2b Kit ELISA, PTGS2 Kit ELISA, Ptgs2 Kit ELISA, LOC100136456 Kit ELISA, ptgs2.S Kit ELISA, ptgs2 Kit ELISA, ptgs2a Kit ELISA, ptgs2b Kit ELISA

Sujet:

Gene Name: Prostaglandin Endoperoxide Synthase 2

Gene Aliases: PGG/HS, PGHS2, PHS2, HCox2, COX2, Cyclooxygenase 2, Prostaglandin G/H Synthase And Cyclooxygenase, Prostaglandin H2 synthase 2

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation