PPM1A Kit ELISA

N° du produit ABIN5658248

Détail du produit PPM1A Kit ELISA

Antigène: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). No significant cross-reactivity or interference between Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) and analogues was observed.

Sensibilité: 0.123 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Autre désignation: Protein Phosphatase, Mg2+/Mn2+ Dependent 1A (PPM1A Produits)

Synonymes: PP2C-ALPHA Kit ELISA, PP2CA Kit ELISA, PP2Calpha Kit ELISA, pp2ca1 Kit ELISA, ppp1r13b Kit ELISA, si:ch211-253p18.1 Kit ELISA, wu:fd42g12 Kit ELISA, zgc:153820 Kit ELISA, 2310003C21Rik Kit ELISA, 2900017D14Rik Kit ELISA, AI427932 Kit ELISA, AU017636 Kit ELISA, MMPa-2 Kit ELISA, MPPa-1 Kit ELISA, Pp2c1 Kit ELISA, pp2c-alpha Kit ELISA, pp2ca Kit ELISA, pp2calpha Kit ELISA, pp2ca2 Kit ELISA, protein phosphatase, Mg2+/Mn2+ dependent 1A Kit ELISA, protein phosphatase, Mg2+/Mn2+ dependent, 1Ab Kit ELISA, protein phosphatase 1A, magnesium dependent, alpha isoform Kit ELISA, protein phosphatase, Mg2+/Mn2+ dependent, 1A Kit ELISA, protein phosphatase, Mg2+/Mn2+ dependent 1A L homeolog Kit ELISA, protein phosphatase, Mg2+/Mn2+ dependent, 1Aa Kit ELISA, PPM1A Kit ELISA, ppm1ab Kit ELISA, Ppm1a Kit ELISA, ppm1a.L Kit ELISA, ppm1aa Kit ELISA

Sujet:

Gene Name: Protein Phosphatase, Mg2+/Mn2+ Dependent 1A

Gene Aliases: PP2C-ALPHA, PP2CA, Phosphatase 2C Alpha, Protein Phosphatase 1A(Formerly 2C)Magnesium-Dependent,Alpha

ID gène: 5494

UniProt: P35813