DROSHA Kit ELISA

N° du produit ABIN5658503

Détail du produit DROSHA Kit ELISA

Antigène: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Ribonuclease III, Nuclear (RNASEN). No significant cross-reactivity or interference between Ribonuclease III, Nuclear (RNASEN) and analogues was observed.

Sensibilité: 0.126 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease III, Nuclear (RNASEN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease III, Nuclear (RNASEN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease III, Nuclear (RNASEN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease III, Nuclear (RNASEN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease III, Nuclear (RNASEN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease III, Nuclear (RNASEN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Autre désignation: Ribonuclease III, Nuclear (DROSHA Produits)

Synonymes: im:7150667 Kit ELISA, zgc:158612 Kit ELISA, ETOHI2 Kit ELISA, HSA242976 Kit ELISA, RANSE3L Kit ELISA, RN3 Kit ELISA, RNASE3L Kit ELISA, RNASEN Kit ELISA, 1110013A17Rik Kit ELISA, AI874853 Kit ELISA, Etohi2 Kit ELISA, Rn3 Kit ELISA, Rnasen Kit ELISA, RGD1307626 Kit ELISA, ribonuclease type III, nuclear Kit ELISA, Ribonuclease 3 Kit ELISA, ribonuclease 3 Kit ELISA, drosha ribonuclease III Kit ELISA, drosha, ribonuclease type III Kit ELISA, rnasen Kit ELISA, Thicy_0885 Kit ELISA, Sinme_0866 Kit ELISA, Isova_2087 Kit ELISA, Sphch_0770 Kit ELISA, DROSHA Kit ELISA, Drosha Kit ELISA

Sujet:

Gene Name: Ribonuclease III, Nuclear

Gene Aliases: RnaseIII, RNASE3L, Rnase-III, Etohi2, DROSHA, RN3, RNASE3-L, Ribonuclease Type III, Double-Stranded RNA-Specific Endoribonuclease

UniProt: Q9NRR4

Pathways: Regulatory RNA Pathways