SEMA3A Kit CLIA

N° du produit ABIN5658691

Détail du produit SEMA3A Kit CLIA

Antigène: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Reactivité: Humain

Méthode de détection: Chemiluminescent

Type de méthode: Sandwich ELISA

Gamme de detection: 156.25 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Semaphorin 3A (SEMA3A). No significant cross-reactivity or interference between Semaphorin 3A (SEMA3A) and analogues was observed.

Sensibilité: 5.38 pg/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The microplate provided in this kit has been pre-coated with an antibody specific to Semaphorin 3A (SEMA3A). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Semaphorin 3A (SEMA3A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Semaphorin 3A (SEMA3A) level in the sample or standard.,

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3A (SEMA3A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Autre désignation: Semaphorin 3A (SEMA3A Produits)

Synonymes: COLL1 Kit CLIA, HH16 Kit CLIA, Hsema-I Kit CLIA, Hsema-III Kit CLIA, SEMA1 Kit CLIA, SEMAD Kit CLIA, SEMAIII Kit CLIA, SEMAL Kit CLIA, SemD Kit CLIA, coll-1 Kit CLIA, Semad Kit CLIA, COLL-1 Kit CLIA, SEMA3A Kit CLIA, Dsema3a Kit CLIA, Sema3A Kit CLIA, sema1a Kit CLIA, sema3a1 Kit CLIA, semaZ1a Kit CLIA, sema1b Kit CLIA, sema3a2 Kit CLIA, semaZ1b Kit CLIA, semaphorin 3A Kit CLIA, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A Kit CLIA, Sema-3a Kit CLIA, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Aa Kit CLIA, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3Ab Kit CLIA, SEMA3A Kit CLIA, Sema3a Kit CLIA, Sema-3a Kit CLIA, sema3aa Kit CLIA, sema3ab Kit CLIA

Sujet:

Gene Name: Semaphorin 3A

Gene Aliases: SEMAL, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SemD, Coll-1, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A

ID gène: 10371

UniProt: Q14563

Pathways: Regulation of Cell Size