SLC30A8 Kit ELISA

N° du produit ABIN5658864

Détail du produit SLC30A8 Kit ELISA

Antigène: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 30 Member 8 (SLC30A8). No significant cross-reactivity or interference between Solute Carrier Family 30 Member 8 (SLC30A8) and analogues was observed.

Sensibilité: 0.057 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 30 Member 8 (SLC30A8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 30 Member 8 (SLC30A8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 30 Member 8 (SLC30A8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 30 Member 8 (SLC30A8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 30 Member 8 (SLC30A8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 30 Member 8 (SLC30A8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Autre désignation: Solute Carrier Family 30 Member 8 (SLC30A8 Produits)

Synonymes: C820002P14Rik Kit ELISA, ZnT-8 Kit ELISA, ZnT8 Kit ELISA, slc30a3 Kit ELISA, znt8 Kit ELISA, ZNT8 Kit ELISA, E130106K10Rik Kit ELISA, Gm212 Kit ELISA, wu:fe49g03 Kit ELISA, RGD1305098 Kit ELISA, solute carrier family 30 member 8 Kit ELISA, solute carrier family 30 (zinc transporter), member 8 Kit ELISA, solute carrier family 30 (zinc transporter), member 8 L homeolog Kit ELISA, solute carrier family 30, member 10 Kit ELISA, SLC30A8 Kit ELISA, Slc30a8 Kit ELISA, slc30a8.L Kit ELISA, slc30a8 Kit ELISA, Slc30a10 Kit ELISA

Sujet:

Gene Name: Solute Carrier Family 30 Member 8

Gene Aliases: ZNT8, Zinc Transporter 8

ID gène: 169026

UniProt: Q8IWU4

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis