TGFBR1 Kit ELISA

N° du produit ABIN5659461

Détail du produit TGFBR1 Kit ELISA

Antigène: TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta Receptor I (TGFbR1). No significant cross-reactivity or interference between Transforming Growth Factor Beta Receptor I (TGFbR1) and analogues was observed.

Sensibilité: 0.117 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta Receptor I (TGFbR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transforming Growth Factor Beta Receptor I (TGFbR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transforming Growth Factor Beta Receptor I (TGFbR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta Receptor I (TGFbR1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta Receptor I (TGFbR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TGFBR1 (Transforming Growth Factor, beta Receptor 1 (TGFBR1))

Autre désignation: Transforming Growth Factor Beta Receptor I (TGFBR1 Produits)

Synonymes: AAT5 Kit ELISA, ACVRLK4 Kit ELISA, ALK-5 Kit ELISA, ALK5 Kit ELISA, LDS1A Kit ELISA, LDS2A Kit ELISA, MSSE Kit ELISA, SKR4 Kit ELISA, TGFR-1 Kit ELISA, aat5 Kit ELISA, acvrlk4 Kit ELISA, alk-5 Kit ELISA, alk5 Kit ELISA, lds1a Kit ELISA, lds2a Kit ELISA, skr4 Kit ELISA, tgfr-1 Kit ELISA, TGFBR1 Kit ELISA, x-trr1 Kit ELISA, AU017191 Kit ELISA, Alk-5 Kit ELISA, TbetaR-I Kit ELISA, TbetaRI Kit ELISA, Alk5 Kit ELISA, Skr4 Kit ELISA, Tgfr-1 Kit ELISA, tbetaR-I Kit ELISA, tgfbr1 Kit ELISA, zgc:123263 Kit ELISA, transforming growth factor beta receptor 1 Kit ELISA, transforming growth factor beta receptor I Kit ELISA, transforming growth factor beta receptor I S homeolog Kit ELISA, transforming growth factor, beta receptor I Kit ELISA, transforming growth factor, beta receptor 1 Kit ELISA, transforming growth factor, beta receptor 1 a Kit ELISA, TGFBR1 Kit ELISA, tgfbr1 Kit ELISA, tgfbr1.S Kit ELISA, Tgfbr1 Kit ELISA, tgfbr1a Kit ELISA

Sujet:

Gene Name: Transforming Growth Factor Beta Receptor I

Gene Aliases: ACVRLK4, AAT5, ALK5, SKR4, TGFR-1, Activin A Receptor Type II-Like Kinase 53 kDa, Activin receptor-like kinase 5, Serine/threonine-protein kinase receptor R4

ID gène: 7046

UniProt: P36897

Pathways: Growth Factor Binding