TSPO Kit ELISA

N° du produit ABIN5659497

Détail du produit TSPO Kit ELISA

Antigène: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Translocator Protein (TSPO). No significant cross-reactivity or interference between Translocator Protein (TSPO) and analogues was observed.

Sensibilité: 0.113 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocator Protein (TSPO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocator Protein (TSPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocator Protein (TSPO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocator Protein (TSPO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocator Protein (TSPO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocator Protein (TSPO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Autre désignation: Translocator Protein (TSPO Produits)

Synonymes: LOC732875 Kit ELISA, LOC100136652 Kit ELISA, LOC100280949 Kit ELISA, BZRP Kit ELISA, TSPO Kit ELISA, Bzrp Kit ELISA, IBP Kit ELISA, PBR Kit ELISA, Tspo1 Kit ELISA, MBR Kit ELISA, PTBZR02 Kit ELISA, Ptbzr Kit ELISA, RATPTBZR02 Kit ELISA, BPBS Kit ELISA, DBI Kit ELISA, PBS Kit ELISA, PKBS Kit ELISA, PTBR Kit ELISA, mDRC Kit ELISA, pk18 Kit ELISA, peripheral-type benzodiazepine receptor Kit ELISA, Peripheral-type benzodiazepine receptor Kit ELISA, putative peripheral-type benzodiazepine receptor Kit ELISA, translocator protein Kit ELISA, translocator protein homolog Kit ELISA, hypothetical protein Kit ELISA, LOC732875 Kit ELISA, MAV_3896 Kit ELISA, xccb100_2086 Kit ELISA, Smp_102510 Kit ELISA, Sros_1911 Kit ELISA, tspo Kit ELISA, LOC100280949 Kit ELISA, PGTG_16395 Kit ELISA, TSPO Kit ELISA, Tspo Kit ELISA

Sujet:

Gene Name: Translocator Protein

Gene Aliases: IBP, PKBS, PBR, pk18, mDRC, MBR, BZRP, DBI, PTBR, Mitochondrial benzodiazepine receptor, Peripheral-Type Benzodiazepine Receptor/Recognition Site

ID gène: 706

UniProt: P30536

Pathways: Cellular Response to Molecule of Bacterial Origin