TRPM6 Kit ELISA

N° du produit ABIN5674672

Détail du produit TRPM6 Kit ELISA

Antigène: TRPM6 (Transient Receptor Potential Cation Channel, Subfamily M, Member 6 (TRPM6))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 27.6 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: TRPM6 (Transient Receptor Potential Cation Channel, Subfamily M, Member 6 (TRPM6))

Autre désignation: Transient Receptor Potential Cation Channel Subfamily M, Member 6 (TRPM6) (TRPM6 Produits)

Synonymes: TRPM6 Kit ELISA, CHAK2 Kit ELISA, HMGX Kit ELISA, HOMG Kit ELISA, HOMG1 Kit ELISA, HSH Kit ELISA, TRPM6/CHAK2 Kit ELISA, transient receptor potential cation channel subfamily M member 6 Kit ELISA, transient receptor potential cation channel, subfamily M, member 6 Kit ELISA, TRPM6 Kit ELISA, Trpm6 Kit ELISA

Sujet: Alternative name: CHAK2, HMGX, HOMG, HSH, Hypomagnesemia,Secondary Hypocalcemia, Channel kinase 2, Melastatin-related TRP cation channel 6

ID gène: 225997

UniProt: Q8CIR4