COL3A1 Kit ELISA

N° du produit ABIN5674676

Détail du produit COL3A1 Kit ELISA

Antigène: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.25 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 14.5 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Autre désignation: Collagen Type III Alpha 1 (COL3a1) (COL3A1 Produits)

Synonymes: EDS4A Kit ELISA, AW550625 Kit ELISA, Col3a-1 Kit ELISA, MMS10-W Kit ELISA, Ms10w Kit ELISA, mKIAA4231 Kit ELISA, col3a1 Kit ELISA, collagen type III alpha 1 chain Kit ELISA, collagen, type III, alpha 1 Kit ELISA, collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) S homeolog Kit ELISA, COL3A1 Kit ELISA, Col3a1 Kit ELISA, col3a1 Kit ELISA, col3a1.S Kit ELISA

Sujet: Alternative name: COL3-A1, COL3A1, EDS4A, Collagen Type III Alpha 1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Collagen Alpha-1(III)chain

ID gène: 12825

UniProt: P08121

Pathways: Autophagy, Growth Factor Binding