TAS1R2 Kit ELISA

N° du produit ABIN5674684

Détail du produit TAS1R2 Kit ELISA

Antigène: TAS1R2 (Taste Receptor, Type 1, Member 2 (TAS1R2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 27 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: TAS1R2 (Taste Receptor, Type 1, Member 2 (TAS1R2))

Autre désignation: Taste Receptor Type 1 Member 2 (TAS1R2) (TAS1R2 Produits)

Synonymes: GPR71 Kit ELISA, T1R2 Kit ELISA, TR2 Kit ELISA, Aldh4a1 Kit ELISA, Gpr71 Kit ELISA, Tr2 Kit ELISA, T1r2 Kit ELISA, taste 1 receptor member 2 Kit ELISA, taste receptor, type 1, member 2 Kit ELISA, TAS1R2 Kit ELISA, Tas1r2 Kit ELISA

Sujet: Alternative name: TR2, GPR71, T1R2, G Protein-Coupled Receptor 71, Sweet taste receptor T1R2

ID gène: 80834

UniProt: Q8TE23