NR1D2 Kit ELISA

N° du produit ABIN5674715

Détail du produit NR1D2 Kit ELISA

Antigène: NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312 ng/mL - 20 ng/mL

Seuil minimal de détection: 0.312 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0108 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2))

Abstract: NR1D2 Produits

Synonymes: BD73 Kit ELISA, EAR-1R Kit ELISA, RVR Kit ELISA, HZF-2 Kit ELISA, Rev-erb Kit ELISA, REV-ERBB Kit ELISA, NR1D2 Kit ELISA, rvr Kit ELISA, bd73 Kit ELISA, hzf2 Kit ELISA, ear-1r Kit ELISA, hs.37288 Kit ELISA, NR1D2-A Kit ELISA, Reverbbeta-A Kit ELISA, reverbb1 Kit ELISA, reverbb Kit ELISA, reverbb2 Kit ELISA, zgc:92019 Kit ELISA, nuclear receptor subfamily 1 group D member 2 Kit ELISA, nuclear receptor subfamily 1, group D, member 2 Kit ELISA, nuclear receptor subfamily 1, group D, member 2a Kit ELISA, nuclear receptor subfamily 1, group D, member 2b Kit ELISA, NR1D2 Kit ELISA, Nr1d2 Kit ELISA, nr1d2 Kit ELISA, nr1d2a Kit ELISA, nr1d2b Kit ELISA

Sujet: Alternative name: HZF2, BD73, EAR-1r, RVR, Rev-ErbA Beta, Orphan nuclear hormone receptor BD73, Rev-erb alpha-related receptor, V-erbA-related protein 1-related

ID gène: 353187

UniProt: Q60674

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway