SDHB Kit ELISA

N° du produit ABIN5674883

Détail du produit SDHB Kit ELISA

Antigène: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0067 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Autre désignation: Succinate Dehydrogenase Complex Subunit B (SDHB) (SDHB Produits)

Synonymes: succinate dehydrogenase 2-3 Kit ELISA, CG3283 Kit ELISA, Dmel\\CG3283 Kit ELISA, Ip Kit ELISA, SDH Kit ELISA, SDH-IP Kit ELISA, SDH-Ip Kit ELISA, SDHb Kit ELISA, sdhB Kit ELISA, CWS2 Kit ELISA, IP Kit ELISA, PGL4 Kit ELISA, SDH1 Kit ELISA, SDH2 Kit ELISA, SDHIP Kit ELISA, 0710008N11Rik Kit ELISA, fj46d06 Kit ELISA, wu:fj46d06 Kit ELISA, succinate dehydrogenase complex iron sulfur subunit B Kit ELISA, succinate dehydrogenase [ubiquinone] iron-sulfur subunit 3 Kit ELISA, Succinate dehydrogenase, subunit B (iron-sulfur) Kit ELISA, succinate dehydrogenase complex subunit B, iron sulfur (Ip) Kit ELISA, succinate dehydrogenase complex, subunit B, iron sulfur (Ip) Kit ELISA, succinate dehydrogenase complex subunit B, iron sulfur (Ip) S homeolog Kit ELISA, SDHB Kit ELISA, SDH2-3 Kit ELISA, SdhB Kit ELISA, sdhb Kit ELISA, Sdhb Kit ELISA, sdhb.S Kit ELISA

Sujet: Alternative name: SDH1, SDH, Ip, Iron Sulfur, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial

ID gène: 6390

UniProt: P21912