SPI1 Kit ELISA

N° du produit ABIN5674925

Détail du produit SPI1 Kit ELISA

Antigène: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.125 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.125 pg/mL

Application: ELISA

Type d'échantillon: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 33.2 pg/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Autre désignation: Spleen Focus Forming Virus Proviral Integration Oncogene (SPI1) (SPI1 Produits)

Synonymes: OF Kit ELISA, PU.1 Kit ELISA, SFPI1 Kit ELISA, SPI-1 Kit ELISA, SPI-A Kit ELISA, Dis-1 Kit ELISA, Dis1 Kit ELISA, Sfpi-1 Kit ELISA, Sfpi1 Kit ELISA, Spi-1 Kit ELISA, Tcfpu1 Kit ELISA, Tfpu.1 Kit ELISA, Pu.1 Kit ELISA, cb1086 Kit ELISA, pu.1 Kit ELISA, pu1 Kit ELISA, sfpi1 Kit ELISA, si:by184l24.2 Kit ELISA, spi1 Kit ELISA, SPI1/PU.1 Kit ELISA, spi1l Kit ELISA, zgc:152991 Kit ELISA, Spi-1 proto-oncogene Kit ELISA, spleen focus forming virus (SFFV) proviral integration oncogene Kit ELISA, Spi-1 proto-oncogene b Kit ELISA, Spi-1 proto-oncogene a Kit ELISA, SPI1 Kit ELISA, Spi1 Kit ELISA, spi1b Kit ELISA, spi1a Kit ELISA

Sujet: Alternative name: OF, PU.1, SFPI1, SPI-1, SPI-A, SPI-1 Proto-Oncogene, Hematopoietic Transcription Factor PU.1, 31 kDa Transforming Protein

ID gène: 6688

UniProt: P17947

Pathways: Stem Cell Maintenance